There are many techniques besides autoclaving that you can employ to sterilize your utensils and materials such as dry heat, microwave oven, and UV radiation. You may also purchase pre-sterilized equipment from scientific supply stores. You can purchase sterile media from UTEX or employ filter-sterilization on your prepared medium.
If a laminar flow hood is not available, a small, closed dead-air space can be used as long as good sterile techniques are utilized.
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