How do I cryopreserve my strain?

Modified on Mon, Sep 30, 2019 at 11:53 AM

When it is not known if a strain can survive cryopreservation, then it may be desirable to test several different protocols simultaneously.

Past experience at UTEX indicates that cultures survive best when frozen in their normal medium with a low concentration of either DMSO (typically 8-12% v/v) or methanol (typically 5% v/v). Strains can also be tested under different conditions such as different concentrations or different kinds of cryoprotective agents (CPAs), different rates of cooling, different culture media, different concentration of algae, etc.

A copy of the UTEX cryopreservation protocol is available here which utilizes a controlled rate freezing canister. 

We have cryopreserved ~2/3 of the strains at UTEX using this procedure with various CPAs and concentrations.

Was this article helpful?

That’s Great!

Thank you for your feedback

Sorry! We couldn't be helpful

Thank you for your feedback

Let us know how can we improve this article!

Select at least one of the reasons
CAPTCHA verification is required.

Feedback sent

We appreciate your effort and will try to fix the article